Figure S1.
Validation of three-cube FRET. Low resting FRETeff between intracellular soluble CFP and soluble YFP (n = 7 cells) or between PHPLCδ1-CFP and soluble YFP (n = 30 cells). High homologous FRETeff between PHPLCδ1-CFP and PHPLCδ1-YFP probes (n = 39) in resting cells with a high concentration of PM PtdIns(4,5)P2.

Validation of three-cube FRET. Low resting FRETeff between intracellular soluble CFP and soluble YFP (n = 7 cells) or between PHPLCδ1-CFP and soluble YFP (n = 30 cells). High homologous FRETeff between PHPLCδ1-CFP and PHPLCδ1-YFP probes (n = 39) in resting cells with a high concentration of PM PtdIns(4,5)P2.

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