Figure 1. Experimental plan to quantify... | Rockefeller University Press

Figure 1.

Experimental plan to quantify the number of open mPT Pores.(A) Energized mitochondria (I), when overloaded with Ca2+, trigger mPTP opening (II), and due to oncotic forces undergo matrix swelling to the point of rupturing the outer mitochondrial membrane (III), then enlarging until the IMM elastic force reaches equilibrium (IV), which can be equilibrated through addition of impermeable (>1.5 kD) PEG. (B) The rate of mitoplast volume change was used to determine the total flux of water, which was then normalized by oncotic pressure. This, divided by the estimated flux per 2 nm mPT pore, yielded the number of pores per mitoplast. Ion conductance was calculated using a value of 1.5 nS per pore. Panels and data are from Neginskaya et al. 2020.

Experimental plan to quantify the number of open mPT Pores. (A) Energized mitochondria (I), when overloaded with Ca²⁺, trigger mPTP opening (II), and due to oncotic forces undergo matrix swelling to the point of rupturing the outer mitochondrial membrane (III), then enlarging until the IMM elastic force reaches equilibrium (IV), which can be equilibrated through addition of impermeable (>1.5 kD) PEG. (B) The rate of mitoplast volume change was used to determine the total flux of water, which was then normalized by oncotic pressure. This, divided by the estimated flux per 2 nm mPT pore, yielded the number of pores per mitoplast. Ion conductance was calculated using a value of 1.5 nS per pore. Panels and data are from Neginskaya et al. 2020.