![Figure 5. I-V curves of WT16F and mutants in symmetrical 140 mM [Cl−] and with 1 mM [Ca2+]i. (A) Averaged I-V curves of Q559E16F (orange), Q559A16F (green), WT16F (black), Q559R16F (pink), and Q559W16F (purple; n = 4–11). (B) Rectification indices of WT16F and various mutants. (C) I-V curves of Q559H16F at pHi 7.4 (black), 6.4 (blue), and 5.4 (red). (D) Averaged rectification indices of Q559H16F at different pHi. *, P < 0.05 and **, P < 0.005 by Student’s t test compared with WT16F at pHi = 7.4.](https://cdn.rupress.org/rup/content_public/journal/jgp/151/4/10.1085_jgp.201812270/7/jgp_201812270_fig5.jpeg?Expires=1767669753&Signature=QWEaveRnjE356youDJ0LV3HlajXVSjOy~hbAJXpxXnN2kHGBSjIelR3RFjOiY-SJmhetxcIMkHVGXOCzQV3GgbAtiGMSO1RwsHP4nIPX46CQNl2m2VsvBDByhDTo5C1WPgg0jWgFYo1ueXurm3emoGUXHk4c2PD3cFRPDmqAYlhFthfeGRVhyjkPD7YxXXsYLEULCTYrlfIoqfZO~dyQlv31Y9y2f7WMhhlmAzimIr~XsPI-2nqmUuls5JbOHb7UOnZHFeLW9ExaEZIiLQyr9wL~3NKMDKDU9gABeW0Xf3lu0PqfIz68hhy3HRgyBfBokOATKFcVFi8VQ-igFA7j7g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 5.
I-V curves of WT16F and mutants in symmetrical 140 mM [Cl−] and with 1 mM [Ca2+]i. (A) Averaged I-V curves of Q559E16F (orange), Q559A16F (green), WT16F (black), Q559R16F (pink), and Q559W16F (purple; n = 4–11). (B) Rectification indices of WT16F and various mutants. (C) I-V curves of Q559H16F at pHi 7.4 (black), 6.4 (blue), and 5.4 (red). (D) Averaged rectification indices of Q559H16F at different pHi. *, P < 0.05 and **, P < 0.005 by Student’s t test compared with WT16F at pHi = 7.4.
