Fluorescence micrographs of the plasma membrane. (a) TIRF image of an intact live PC12 cell expressing clathrin light chain (CLC; green) and FCHO2 (red) and the overlay image (Larson et al., 2014). (b) Zoomed images from white boxes in panel a. (c) TIRF-SIM image of intact HeLa cell expressing clathrin (green) and FCHO2 (red) and the overlay image (Sochacki et al., 2017). (d) Superresolution direct stochastic optical reconstruction microscopy (dSTORM) localization image of Alexa Fluor 647/nanobody–labeled FCHO2 expressed in an unroofed HeLa cell (Sochacki et al., 2017).