Effects of C-terminal truncation on the voltage dependence of activation and inactivation. (A) A cartoon of NaVAb showing the different truncations of the C-terminal tail domain. (B) G–V curves of NaVAb/WT and C-terminal truncated constructs: NaVAb/WT, V1/2 = −97 ± 1.4 mV (black, n = 7); NaVAbΔ40, V1/2 = −110.7 ± 2.1 mV (red, n = 5); NaVAbΔ28, V1/2 = −90.1 ± 1.5 mV (blue, n = 8); NaVAbΔ10, V1/2 = −90.7 ± 0.8 mV (green, n = 4); NaVAb/Δ7, V1/2 = -104 ± 2 mV (gold, n = 5); and NaVAbΔ3, V1/2 = −89.1 ± 0.8 mv (gray, n = 4) (C) Steady-state inactivation curves of the constructs. NaVAb/WT, V1/2 = −119 ± 1.1 mV (black, n = 5); NaVAbΔ40, V1/2 = −116.1 ± 4.4 mV (red, n = 5); NaVAbΔ28, V1/2 = −106.7 ± 0.6 (blue, n = 3); NaVAbΔ10, V1/2 = −109.7 ± 0.6 mV (green, n = 5); NaVAbΔ7, V1/2 = −104.7 ± 0.4 mV (gold, n = 3); and NaVAbΔ3, V1/2= −121.7 ± 0.4 (gray, n = 5). Error bars represent SEM.
