Lymphatic contractile function with the Ano1 inhibitor benzbromarone. (A–C) Representative inner diameter trace from a single IALV during the pressure step protocol (A), and at pressures 2–5 cm H₂O with 7 µM benzbromarone (B) and washout (C). (B) Both a single contraction (^) or occasionally two consecutive contractions (%) were routinely observed in 7 µM benzbromarone. Statistical comparison of summary data for the contractile parameters frequency (D), amplitude (E), and tone (F). (G) Representative trace of c-KitCreER^T2-Ano1KO IALV in response to 7 µM benzbromarone. *, P < 0.05 for benzbromarone versus Krebs with n = 7.