Figure 7. Inhibition of LMC IClCa and effect of benzbromarone dose-response on IALV contractile parameters. (A) Representative trace of absolute Cl− current during a voltage ramp protocol using conventional whole-cell patch clamp of freshly dispersed LMCs in response to the Ano1 inhibitor benzbromarone and after washout (200 nM Ca2+ in patch pipette). Summary of current density at −80 mV (B) and +80 mV (C). n = 23 LMCs for control and 10 µM benzbromarone; n = 10 LMCs whose patch integrity persisted through washout (D). Representative inner diameter trace of a WT IALV pressurized to 3 cm H2O in response different concentrations of benzbromarone (1, 3, 5, 7, and 10 µM). Summary of contractile parameters frequency (E), amplitude (F), and tone (G). *, P < 0.05 when compared with Krebs control with n = 4.
Figure 7.

Inhibition of LMC IClCa and effect of benzbromarone dose-response on IALV contractile parameters. (A) Representative trace of absolute Cl− current during a voltage ramp protocol using conventional whole-cell patch clamp of freshly dispersed LMCs in response to the Ano1 inhibitor benzbromarone and after washout (200 nM Ca2+ in patch pipette). Summary of current density at −80 mV (B) and +80 mV (C). n = 23 LMCs for control and 10 µM benzbromarone; n = 10 LMCs whose patch integrity persisted through washout (D). Representative inner diameter trace of a WT IALV pressurized to 3 cm H2O in response different concentrations of benzbromarone (1, 3, 5, 7, and 10 µM). Summary of contractile parameters frequency (E), amplitude (F), and tone (G). *, P < 0.05 when compared with Krebs control with n = 4.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal