Figure 5. Inducible deletion of Ano1 from LMCs reduces contraction frequency and abrogates pressure-dependent contraction frequency. (A) Representative trace of Cl− current density during a voltage ramp protocol using conventional whole-cell patch clamp of freshly dispersed LMCs from IALV isolated from Ano1fl/fl (n = 8) or SMMHC-CreERT2-Ano1KO (n = 8) mice (1 µM Ca2+ in patch pipette). Summary of current density at −80 mV (B) and +80 mV (C). (D) Normalized Ano1 expression determined by qPCR from whole IALV in Ano1fl/fl controls (n = 5) and SMMHC-CreERT2-Ano1KO (n = 4) mice. 20-s clips from a single representative inner diameter trace over the pressure step protocol for an IALV isolated from the Ano1fl/fl controls (E) and the SMMHC-CreERT2-Ano1KO mice (F). Statistical summary of contractile parameters frequency (G), amplitude (H), and tone (I) at each pressure. *, P < 0.05 for SMMHC-CreERT2-Ano1KO (n = 27) versus Ano1fl/fl controls (n = 22).
Figure 5.

Inducible deletion of Ano1 from LMCs reduces contraction frequency and abrogates pressure-dependent contraction frequency. (A) Representative trace of Cl− current density during a voltage ramp protocol using conventional whole-cell patch clamp of freshly dispersed LMCs from IALV isolated from Ano1fl/fl (n = 8) or SMMHC-CreERT2-Ano1KO (n = 8) mice (1 µM Ca2+ in patch pipette). Summary of current density at −80 mV (B) and +80 mV (C). (D) Normalized Ano1 expression determined by qPCR from whole IALV in Ano1fl/fl controls (n = 5) and SMMHC-CreERT2-Ano1KO (n = 4) mice. 20-s clips from a single representative inner diameter trace over the pressure step protocol for an IALV isolated from the Ano1fl/fl controls (E) and the SMMHC-CreERT2-Ano1KO mice (F). Statistical summary of contractile parameters frequency (G), amplitude (H), and tone (I) at each pressure. *, P < 0.05 for SMMHC-CreERT2-Ano1KO (n = 27) versus Ano1fl/fl controls (n = 22).

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