Figure 3. The IALV LMC CaCC is not cGMP-dependent. (A) Representative RT-PCR for bestrophin 1, bestrophin 2, CLCA1, and CLCA3 expression in IALV. (B) Representative Cl− current density trace in response to a voltage ramp protocol during whole-cell conventional patch clamp of freshly dispersed IALV LMCs (n = 6), in response to stimulation with 300 nM 8-br-cGMP (n = 6), and 10 µM ZnCl2 (n = 4). Summary of current density at −80 mV (C) and +80 mV (D). Representative RT-PCR for NKCC1 (E) and KNCC2 (F) in IALVs and LMCs purified from IALVs with kidney extract as a positive control.
Figure 3.

The IALV LMC CaCC is not cGMP-dependent. (A) Representative RT-PCR for bestrophin 1, bestrophin 2, CLCA1, and CLCA3 expression in IALV. (B) Representative Cl− current density trace in response to a voltage ramp protocol during whole-cell conventional patch clamp of freshly dispersed IALV LMCs (n = 6), in response to stimulation with 300 nM 8-br-cGMP (n = 6), and 10 µM ZnCl2 (n = 4). Summary of current density at −80 mV (C) and +80 mV (D). Representative RT-PCR for NKCC1 (E) and KNCC2 (F) in IALVs and LMCs purified from IALVs with kidney extract as a positive control.

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