IALV LMCs display a functional CaCC current. (A) Representative recordings of whole cell patch clamp experiments designed to assess Cl⁻ current of freshly dissociated LMCs with either low Ca²⁺ (200 nM, black trace, n = 16 LMCs) or high Ca²⁺ (1 µM, red trace, n = 8 LMCs) in the patch pipette. Statistical analysis of current density at both −80 mV (B) and +80 mV (C). Representative traces showing current reversal potential with Krebs bath solution 135 mM Cl⁻ (n = 9) substituted with either glutamate (86 mM glutamate and 38 mM Cl⁻, n = 11) or iodide (125 mM I⁻ and 10 mM Cl⁻, n = 8). Representative raw traces in each buffer (D) and the summary data (E). Representative current trace in response to a voltage ramp protocol during conventional whole-cell patch clamp with or without 1 µM nifedipine present to block Ca²⁺ entry through L-type channels (F). A summary of recorded currents at −80 mV (G) and +80mV (H) with or without nifedipine (n = 8 cells). pA, picoamperes; pF, picofarad. *, P < 0.05.