S2030 ablation generates adaptive changes at RyR2-S2808 and S2814 phosphorylation sites in mutant cells. (A) Representative blot showing the phosphorylation of RyR2 at the S2808 and S2814 sites in both WT and S2030A^+/+ cells in control (C) and during Iso stimulation. (B and C) The level of the phosphorylation was expressed as the ratio of the respective phospho- and total RyR ODs, both normalized to the GAPDH signal (N = 8 mice for both WT and S2030A^+/+ cells were used to compare baseline phosphorylation; between these, N = 4 animals were also assessed after Iso application). These data were tested for paired statistical difference. *, P < 0.05 versus control; †, P < 0.05 versus WT. The whiskers cover the range from 10% to 90%.