Figure 4.
Figure 4. SERT-mediated single channel currents. Channel activities from oocytes expressing the wild-type (A) and N177G mutant (B) rat SERT in outside-out patches at −100 mV. Traces labeled Na and Li were recorded in the absence of 5-HT, showing the leakage states. Traces labeled Na + 5-HT represent the channel activities induced by 5-HT (10 mM), showing the 5-HT–induced state. The channel activities were blocked by the uptake inhibitor fluoxetine (10 mM). 5-HT–induced channel activities require Na+ as a permeable ion; only a few openings are observed in the presence of Li+ plus 5-HT. All traces in A are from a single patch; those in B are from another patch. Both experiments were performed with normal Cl− concentrations on both sides of the membrane. The single-channel conductance was much larger for the SERT N177G mutant, demonstrating that they originated from SERT. Figure 4 is reprinted from Lin et al. (1996) with permission from Biophysical Journal.

SERT-mediated single channel currents. Channel activities from oocytes expressing the wild-type (A) and N177G mutant (B) rat SERT in outside-out patches at −100 mV. Traces labeled Na and Li were recorded in the absence of 5-HT, showing the leakage states. Traces labeled Na + 5-HT represent the channel activities induced by 5-HT (10 mM), showing the 5-HT–induced state. The channel activities were blocked by the uptake inhibitor fluoxetine (10 mM). 5-HT–induced channel activities require Na+ as a permeable ion; only a few openings are observed in the presence of Li+ plus 5-HT. All traces in A are from a single patch; those in B are from another patch. Both experiments were performed with normal Cl− concentrations on both sides of the membrane. The single-channel conductance was much larger for the SERT N177G mutant, demonstrating that they originated from SERT. Figure 4 is reprinted from Lin et al. (1996) with permission from Biophysical Journal.

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