Figure 1.
Figure 1. Photoresponses of mouse cones recorded with whole-cell patch clamp. (A) Current responses recorded from a WT mouse cone with minimal rod input. The soma of the cone was voltage clamped and held at −50 mV. Brief flashes of increasing strength (22–2,100 P*/flash) were presented at time = 0 ms. (B) Voltage responses from the same cone as A, stimulated with the same light flashes. Data traces in A and B are averages of seven presentations for each of the flashes and were filtered digitally during analysis (50 Hz). (C) An individual cone visualized with a far-red dye (Alexa Fluor 750) perfused into the cell from the recording pipette. (D) Merged image of C (pseudocolored red) and wide-field image of the same retinal slice. Scale bar, 20 µm. Refer to the image caption for details.

Photoresponses of mouse cones recorded with whole-cell patch clamp. (A) Current responses recorded from a WT mouse cone with minimal rod input. The soma of the cone was voltage clamped and held at −50 mV. Brief flashes of increasing strength (22–2,100 P*/flash) were presented at time = 0 ms. (B) Voltage responses from the same cone as A, stimulated with the same light flashes. Data traces in A and B are averages of seven presentations for each of the flashes and were filtered digitally during analysis (50 Hz). (C) An individual cone visualized with a far-red dye (Alexa Fluor 750) perfused into the cell from the recording pipette. (D) Merged image of C (pseudocolored red) and wide-field image of the same retinal slice. Scale bar, 20 µm.

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