Identification of cellular subcompartments. (A and B) HEK cells were loaded with CoroNaGreen-AM in ACSF. (A) FL image; dark pixels represent short FL, bright pixels long FL. (B) Corresponding image showing the emission intensity of CoroNaGreen. (C) Same field of view, showing the cells labeled with Mitotracker. (D and E) Merge (white) of Mitotracker fluorescence (magenta) with CoroNaGreen FL (cyan, D) or CoroNaGreen intensity (cyan, E). Scale bar, 20 µm.