Figure 8.
Figure 8. Effect of deglycosylation on the binding of pore blockers. (A and B) Current traces represent ionic currents in response to voltage steps to −50, −40, 0, and +40 mV from a holding potential of −80 mV. Tail currents shown were in response to voltage of −50 mV. A compares the inhibition by 100 nM AgTx before (top) and after (bottom) PNGase F treatment (n = 2). At +40 mV, AgTx blocked 48% of the ionic current before and 62% after treatment, respectively. At 0 mV, it inhibited 48% of the ionic current before and 63% treatment, respectively. B compares the inhibition by 0.1 mM 4AP before (top) and after (bottom) PNGase F treatment (n = 2). At +40 mV, 4AP blocked 55% of the ionic current before and 53% after treatment, respectively. At 0 mV, it inhibited 48% of the ionic current before and 50% after treatment, respectively. These experiments were performed by using the two-microelectrode voltage-clamp technique. DeGly, deglycosylation.

Effect of deglycosylation on the binding of pore blockers. (A and B) Current traces represent ionic currents in response to voltage steps to −50, −40, 0, and +40 mV from a holding potential of −80 mV. Tail currents shown were in response to voltage of −50 mV. A compares the inhibition by 100 nM AgTx before (top) and after (bottom) PNGase F treatment (n = 2). At +40 mV, AgTx blocked 48% of the ionic current before and 62% after treatment, respectively. At 0 mV, it inhibited 48% of the ionic current before and 63% treatment, respectively. B compares the inhibition by 0.1 mM 4AP before (top) and after (bottom) PNGase F treatment (n = 2). At +40 mV, 4AP blocked 55% of the ionic current before and 53% after treatment, respectively. At 0 mV, it inhibited 48% of the ionic current before and 50% after treatment, respectively. These experiments were performed by using the two-microelectrode voltage-clamp technique. DeGly, deglycosylation.

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