Figure 6.
Figure 6. Effect of deglycosylation on ionic currents from the ILT mutant Shaker KV channel. (A) Normalized ionic current traces elicited from a holding potential of −100 mV to +100 mV (left) and +120 mV (right), before (black) and after (red) PNGase F treatment. (B) Normalized tail currents before (black) and after (red) PNGase F treatment upon return from the voltage step to +120 mV. Left: Zoomed-in traces depicted in A (+120 mV). Right: Traces from a different oocyte. Ionic currents from ILT channels were P/6 subtracted with subpulses of opposite polarity from the holding potential. These experiments were performed by using the cut-open oocyte voltage clamp. DeGly, deglycosylation.

Effect of deglycosylation on ionic currents from the ILT mutant Shaker KV channel. (A) Normalized ionic current traces elicited from a holding potential of −100 mV to +100 mV (left) and +120 mV (right), before (black) and after (red) PNGase F treatment. (B) Normalized tail currents before (black) and after (red) PNGase F treatment upon return from the voltage step to +120 mV. Left: Zoomed-in traces depicted in A (+120 mV). Right: Traces from a different oocyte. Ionic currents from ILT channels were P/6 subtracted with subpulses of opposite polarity from the holding potential. These experiments were performed by using the cut-open oocyte voltage clamp. DeGly, deglycosylation.

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