Figure 2.
Figure 2. Effects of deglycosylation on the ionic currents carried by Shaker KV channels. (A and B) Ionic currents from an oocyte expressing Shaker KV channels before (A) and after (B) 5-min exposure to PNGase F. Ionic currents were recorded by using the two-microelectrode voltage-clamp technique. These currents were elicited by 50-ms voltage steps from a holding potential of −80 to +40 mV, every 10 mV. (C) Time rise from 10% to 90% current activation. (D) Relative conductance plots before (black) and after (red) PNGase F treatment. Error bars in C and D represent mean ± SEM, (n = 5). DeGly, deglycosylation.

Effects of deglycosylation on the ionic currents carried by Shaker KV channels. (A and B) Ionic currents from an oocyte expressing Shaker KV channels before (A) and after (B) 5-min exposure to PNGase F. Ionic currents were recorded by using the two-microelectrode voltage-clamp technique. These currents were elicited by 50-ms voltage steps from a holding potential of −80 to +40 mV, every 10 mV. (C) Time rise from 10% to 90% current activation. (D) Relative conductance plots before (black) and after (red) PNGase F treatment. Error bars in C and D represent mean ± SEM, (n = 5). DeGly, deglycosylation.

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