Figure 2. Purification of an inhibitor... | Rockefeller University Press

Figure 2.

$Figure 2. Purification of an inhibitor against Kir6.2. (A and B) Currents of Kir6.2 coexpressed with SUR1, recorded in the absence (black trace) or the presence (red trace) of 1:2,000 diluted venom (A) or ∼30 nM purified inhibitor from the venom (B). The dotted line indicates the zero-current level. (C) HPLC chromatograph (25-cm-long C18 column) of crude venom, where the sample was eluted with a linear methanol gradient of 1% per min at a 1-ml/min flow rate. The active peak is indicated by the arrow. (D) HPLC chromatograph (15-cm-long C18 column) of the active fraction collected from C, where sample was eluted with a methanol gradient of 0.2% per min at a 0.2-ml/min flow rate.$

Purification of an inhibitor against Kir6.2. (A and B) Currents of Kir6.2 coexpressed with SUR1, recorded in the absence (black trace) or the presence (red trace) of 1:2,000 diluted venom (A) or ∼30 nM purified inhibitor from the venom (B). The dotted line indicates the zero-current level. (C) HPLC chromatograph (25-cm-long C18 column) of crude venom, where the sample was eluted with a linear methanol gradient of 1% per min at a 1-ml/min flow rate. The active peak is indicated by the arrow. (D) HPLC chromatograph (15-cm-long C18 column) of the active fraction collected from C, where sample was eluted with a methanol gradient of 0.2% per min at a 0.2-ml/min flow rate.