Ru360 inhibition of I_MCU. (A) Left: I–V relation of I_MCU in the presence of 20 mM Ca²⁺ and various concentrations of Ru360. Right: currents at −120 mV plotted in a time course. (B) A Ru360 dose-response curve. Data fitting uses a standard single-site binding model (red curve). Each data point represents the mean of at least five independent repeats. Data are presented as mean ± SEM. (C) Current (−120 mV) recovery upon Ru360 (500 nM) removal follows a single exponential curve (red). The voltage protocol is the same as in Fig. 6.