Figure 3.
Figure 3. Effect of different combinations of liganded subunits on steady-state activation. Eight tetrameric and one dimeric concatemer are compared. cAMP is applied at a saturating concentration of 50 µM. (A) cAMP-induced voltage shift ΔVh (mV), determined as described for Fig. 2A by using Eq. 1. The amount of voltage shift by liganded subunits is additive. In channels with two functional CNBDs, the cis and the trans position are indistinguishable. (B) Effect of saturating cAMP on current increase at the saturating voltage of −150 mV, ΔI−150mV. Two liganded subunits suffice to generate the maximum effect. Data points contain 8–17 recordings. Error bars indicate SEM.

Effect of different combinations of liganded subunits on steady-state activation. Eight tetrameric and one dimeric concatemer are compared. cAMP is applied at a saturating concentration of 50 µM. (A) cAMP-induced voltage shift ΔVh (mV), determined as described for Fig. 2A by using Eq. 1. The amount of voltage shift by liganded subunits is additive. In channels with two functional CNBDs, the cis and the trans position are indistinguishable. (B) Effect of saturating cAMP on current increase at the saturating voltage of −150 mV, ΔI−150mV. Two liganded subunits suffice to generate the maximum effect. Data points contain 8–17 recordings. Error bars indicate SEM.

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