Impact of MB-001 and fenretinide administration on wild-type mouse scotopic ERG responses. (A) Representative dark-adapted ERGs obtained from 7-mo-old C57BL/6J mice treated with vehicle and bleached, treated with MB-001 and bleached, or treated with MB-001 and not bleached. Animals were dark adapted for 6 h before ERG recordings. Flash strength is color coded: black, −3.6 log cd·s/m2; blue, −2.4 log cd·s/m2; light purple, −1.2 log cd·s/m2; purple, 0 log cd·s/m2; red, 1.4 log cd·s/m2. (B and C) Dark-adapted a-wave and b-wave responses for the three treatment groups. Note that MB-001 reduced a- and b-wave ERG amplitude recovery both after a bleach and, to a lesser extent, in mice reared under standard vivarium lighting conditions. Data points indicate means (±SEM) of five mice per group for the vehicle and bleached, MB-001–treated groups and three mice for the nonbleached, MB-001–treated group. (D) Representative dark-adapted ERGs obtained from 2-mo-old C57BL/6J mice administered vehicle and bleached or administered fenretinide and bleached. Animals were dark adapted for 6 h before ERG recordings. Flash strength is color coded: black, −3.6 log cd·s/m2; blue, −2.4 log cd·s/m2; light purple, −1.2 log cd·s/m2; purple, 0 log cd·s/m2; red, 1.4 log cd·s/m2. (E and F) Scotopic a-wave and b-wave responses for the two treatment groups. Data points indicate means (±SEM) of three mice per group. Fenretinide treatment reduced scotopic b-wave amplitudes slightly, whereas a-wave responses were unaffected.
