Cell volume-sensitive currents induced by the homomeric 8C-8A(IL) chimera. (A) Properties of VRAC currents observed in Lrrc8^−/− 8C-8A(IL)-expressing HCT116 cells with stable resting currents. Left and middle panels show time course and current–voltage relationships of resting, swelling-activated, and shrinkage-inactivated currents. Values are means ± SEM (n = 4–5). Right panel shows representative traces of swelling-activated currents induced by the 8C-8A(IL) chimera. (B) Properties of spontaneously activating currents in Lrrc8^−/− HCT116 cells expressing the 8C-8A(IL) chimera. Left panel shows time course of shrinkage-induced inactivation of the spontaneously activated current and reactivation by cell swelling. Middle panel shows current–voltage relationships of currents observed under isotonic conditions after cell shrinkage and reactivation by cell swelling. Values are means ± SEM (n = 3). Representative traces (right panel) of spontaneous and swelling-activated 8C-8A(IL) chimera-induced current observed in the same cell.