The relationships between pHt and pHcyto and between NHE flux and pHcyto in polarizing, K-based solutions. (A) The steady-state pHt versus pHcyto in high [K+]cyto–based solutions in all 10 fibers investigated. (B) Result from three fibers with multiple measurements at different pHcyto plotted as means ± SEM. (C) NHE-proton flux normalized to the response in pH-6.8 K-based solution derived from the results shown in B using Eq. 20 (predicted) and expected curve for NHE proton flux based on Eq. 15, normalized to the value in pH-6.8 K-based solution (Cha’s model). Results are means ± SEM. Note that the (predicted) normalized ΦH-NHE fluxes for individual fibers changes little if the Vm value is between −75 and −90 mV.