Protein kinase inhibition in intact cells reproduces the gating changes during run-down in dialyzed cells. (A) Voltage dependence of activation (right) and prepulse inactivation (left), determined at the onset of run-down (time 0) and in 7-min intervals thereafter in nine cells pretreated with 1 µM stauro for 30–60 min. (B) Comparison of half-activation voltages at the onset of run-down in cells pretreated with 1 µM stauro (same cells as in A) and under control conditions (same cells as in Fig. 4). (C) Comparison of half-inactivation voltages at the onset of run-down in cells pretreated with 1 µM stauro and under control conditions (same cells as in B). (D) Time-course of changes in half-activation voltages during run-down observed in cells pretreated with 1 µM stauro and under control conditions (same cells as in B). (E) Time course of changes in half-inactivation voltages during run-down observed in cells pretreated with 1 µM stauro and under control conditions (same cells as in B). (F) Time course of changes in activation slope factors during run-down observed in cells pretreated with 1 µM stauro and under control conditions (same cells as in B). ***, P < 0.001 vs. Ctrl (one-way ANOVA with Bonferroni post hoc correction). Values are expressed as mean ± SEM.