ATP protection from run-down involves serine/threonine phosphorylation. (A) Mean data from 10 recordings with 5 mM MgATP and 10 µM stauro (ATP+Stauro), 4 recordings with 10 µM stauro only (Stauro), and 12 parallel control recordings (Ctrl). (B) Comparison of the residual IBa after 7 min of run-down in recordings with Stauro or ATP+Stauro and in parallel control recordings (same cells as in A). (C) Comparison of the run-up duration in recordings with Stauro or ATP+Stauro and in parallel control recordings (same cells as in A). (D) Mean data from seven recordings after pretreatment with 1 µM stauro (Stauro PT) and five parallel control recordings (Ctrl). Note that current amplitudes are expressed in pA/pF to illustrate the reduced peak current densities after stauro pretreatment. (E) Comparison of the initial peak current densities observed after stauro pretreatment and in parallel control recordings (same cells as in D). (F) Comparison of the maximum peak current densities observed after stauro pretreatment and in parallel control recordings (same cells as in D). (G) Same data as in D but normalized to the initial current amplitudes to illustrate changes in the magnitude of run-up. (H) Comparison of the current increase during run-up after stauro pretreatment and in parallel control recordings (same cells as in D). (I) Comparison of the run-up duration after stauro pretreatment and in parallel control recordings (same cells as in D). *, P < 0.05; **, P < 0.01 (one-way ANOVA with Bonferroni post hoc correction). Values are expressed as mean ± SEM.
