Overview of changes in run-up and run-down observed under various experimental conditions. Data are from the same cells as in Figs. 9, 11, and 13 with additional data from six recordings with 5 mM MgATP + 100 µM GS (+GS) and four parallel control recordings and three recordings after a 60-min pretreatment with 10 µM DM and five parallel control recordings. For comparison between the different experimental conditions, all values obtained with a given test solution were normalized as described in Fig. 7 and Materials and methods. Symbols for statistical significance refer to the comparison of normalized values between different experimental conditions. (A) Comparison of mean changes relative to parallel control recordings in the duration of run-up (Δ Time to Imax). (B) Comparison of mean changes relative to parallel control recordings in the magnitude of run-up (Δ Increase). (C) Comparison of mean changes relative to parallel control recordings in the residual current after 7 min of run-down (Δ Remaining IBa). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (one-way ANOVA with Bonferroni post hoc correction in A and C or Welch’s ANOVA with the Games-Howell multiple-comparison method in B). Values are expressed as mean ± SEM.
