ATP protection from run-down is not related to increased PIP2 synthesis. (A) Mean data from seven recordings with 5 mM MgATP and 10 µM WM (ATP + WM), nine recordings with 5 mM MgATP after WM pretreatment (ATP + WM PT), and 13 parallel control recordings. (B) Comparison of the residual IBa after 7 min of run-down under control conditions, with ATP and WM and with ATP after WM pretreatment (same cells as in A). (C) Comparison of the run-up duration under control conditions, with ATP and WM and with ATP after WM pretreatment (same cells as in A). (D) Comparison of the current increase under control conditions, with ATP and WM and with ATP after WM pretreatment (same cells as in A). (E) Mean data from five recordings with 1 mg/ml BSA and nine parallel control recordings. (F) Mean data from six recordings with 5 µM U73122 and eight parallel control recordings. **, P < 0.01; ***, P < 0.001 vs. Ctrl (one-way ANOVA with Bonferroni post hoc correction). Values are expressed as mean ± SEM.
