Hydrolysable ATP stabilizes Cav2.3 channel gating and function. (A) Mean IV relationships measured at the onset of run-down and in 7-min intervals thereafter with a pipette solution containing 5 mM MgATP (n = 6). (B) Voltage dependence of activation (right) and prepulse inactivation (left), determined at the same time points as in A (same cells as in A). (C) Time course of changes in the half-activation voltage during run-down observed in the absence (black squares, same cells as in Fig. 4) and presence (orange circles, same cells as in A) of 5 mM MgATP. (D) Time course of changes in the half-inactivation voltage during run-down observed in the absence (black squares, same cells as in Fig. 4) and presence (orange circles, same cells as in A) of 5 mM MgATP. (E) Time course of changes in the inactivation slope factor during run-down observed in the absence (black squares, same cells as in Fig. 4) and presence (orange circles, same cells as in A) of 5 mM MgATP. Values are expressed as mean ± SEM.
