Overview of changes in run-up and run-down observed under various experimental conditions. Data are from the same cells as in Figs. 5 and 6 with additional data from 7 recordings with cytosolic extract (Cytosol) and 6 parallel control recordings, 9 recordings with 5 mM NaATP and 7 parallel control recordings, and 13 recordings performed with Ca2+ as the charge carrier and 5 mM MgATP in the presence or absence of 3 µM CaM plus 5 parallel control recordings. For comparison between the different experimental conditions, all values obtained with a given test solution were normalized by subtracting the mean value observed in parallel control recordings and then averaged, so the derived parameters provide a measure for the mean change relative to control (for details see Materials and methods). Symbols for statistical significance refer to the comparison of normalized values between different experimental conditions. (A) Comparison of mean changes relative to parallel control recordings in the duration of run-up (Δ Time to Imax). (B) Comparison of mean changes relative to parallel control recordings in the magnitude of run-up (Δ Increase). (C) Comparison of mean changes relative to parallel control recordings in the residual current after 7 min of run-down (Δ Remaining IBa). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (one-way ANOVA with Bonferroni post hoc correction in A and C or Welch’s ANOVA with the Games-Howell multiple-comparison method in B). Values are expressed as mean ± SEM.
