ATP protection from run-down is reproduced by perforated-patch recordings. Comparison of time-course recordings with 40 µM β-escin in the absence and presence of MgATP. (A) Mean data from 11 perforated-patch recordings with standard internal solution (orange diamonds), 6 perforated-patch recordings with 5 mM MgATP (orange circles), and 3 perforated-patch recordings with standard internal solution in which the patch ruptured during perforation (open squares). All recordings were performed with 40 µM β-escin as the perforating agent. (B) Comparison of the residual IBa after 7 min of run-down in recordings with patch rupture and in perforated recordings without or with 5 mM MgATP (same cells as in A). (C) Comparison of the run-up duration in recordings with patch rupture and in perforated recordings without or with 5 mM MgATP (same cells as in A). (D) Comparison of the current increase in recordings with patch rupture and in perforated recordings without or with 5 mM MgATP (same cells as in A). *, P < 0.05; **, P < 0.01 vs. Ctrl (one-way ANOVA with Bonferroni post hoc correction). Values are expressed as mean ± SEM.
