Hydrolysable ATP provides protection from Cav2.3 channel run-down. Comparison between time-course recordings performed with the indicated test substances and parallel control recordings with standard internal solution. (A) Mean data from 5 recordings with 1 mM MgATP (orange squares), 8 recordings with 5 mM MgATP (orange circles), and 13 parallel control recordings (open squares). (B) Comparison of the residual IBa after 7 min of run-down under control conditions and with 1 or 5 mM MgATP in the pipette solution (same cells as in A). (C) Comparison of the run-up duration under control conditions and with 1 or 5 mM MgATP in the pipette solution (same cells as in A). (D) Comparison of the current increase under control conditions and with 1–5 mM MgATP in the pipette solution (same cells as in A). (E) Mean data from five recordings with 1 mM MgCl2 (orange squares) and seven parallel control recordings (open squares). (F) Mean data from six recordings with 0.3 mM MgGTP (orange squares) and five parallel control recordings (open squares). (G) Mean data from eight recordings with 5 mM AMP-PNP (orange squares) and seven parallel control recordings (open squares). **, P < 0.01; ***, P < 0.001 vs. Ctrl (one-way ANOVA with Bonferroni post hoc correction). Values are expressed as mean ± SEM.
