Evolution and run-down of Ca_v2.3 channel currents carried by 5 mM Ba²⁺. (A) Time course of changes in peak whole-cell currents evoked by repetitive step depolarization to 10 mV every 30 s (n = 10). For consistency with the later figures, time 0 is defined as the time of maximum inward current (I_max). Gray lines in the background indicate time course of changes in individual recordings. Also shown are mean current traces corresponding to the time points indicated next to the traces and by filled symbols in the graph. (B) Same data as in A but normalized to the maximum peak current amplitude (I_max). Gray lines in the background indicate the time course of changes in individual recordings. Also shown above is the lack of spontaneous changes in R_s over the time course of the recordings. (C) Same current traces as in A but scaled to their maximum amplitude to visualize changes in their shape. Values are expressed as mean ± SEM.