Figure 6.
![Figure 6. Competitive inhibition of thiamine transport by PnuTSw reconstituted in proteoliposomes by thiamine analogues. (a) [3H]thiamine uptake in the presence of 1 mM of thiamine analogues. TMP (open triangles), TPP (open circles), oxythiamine (black inverted triangles), and pyrithiamine (black squares). Positive control with no additions (black circles). (b) Counter flow of [3H]thiamine into proteoliposomes loaded with 1 mM thiamine (black inverted triangles), pyrithiamine (black circles), oxythiamine (open circles), TMP (open triangles), and TPP (black squares). All error bars indicate the SD from three experiments.](https://cdn.rupress.org/rup/content_public/journal/jgp/150/1/10.1085_jgp.201711850/5/jgp_201711850_fig6.jpeg?Expires=1768629677&Signature=rVAVfRZALPex~azRq36uefVPMXIPJZPdMKkZMm98liLI0qq7Zdq~P1XON63KH6pacXoPyWqKIRdZrDoRx9AYqB2qNsDRc2GtkfI2JgGOpIrYUVBkNJP8BMNE1Ds~qpfd5UseGjQclNHr7fi3N~PSUsg6Q0cwK472YoIto~mHcf3Ic0A6l1HR8whCxmGGcLxmmJYsdL0N4k5-3j9Br8DnzzfwiuWjNAxdTRqZmjZyuMt~S0NUsBY0cedZyeOXaRPVdNnLXyWSj87sCakqhfA4fVrPRDYGZdArnsiJ1EKbWRLnRCSMdCe3kebSpPvZlTZoz-VZ-qAJ-USUy9mf1x9QZw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Competitive inhibition of thiamine transport by PnuTSw reconstituted in proteoliposomes by thiamine analogues. (a) [3H]thiamine uptake in the presence of 1 mM of thiamine analogues. TMP (open triangles), TPP (open circles), oxythiamine (black inverted triangles), and pyrithiamine (black squares). Positive control with no additions (black circles). (b) Counter flow of [3H]thiamine into proteoliposomes loaded with 1 mM thiamine (black inverted triangles), pyrithiamine (black circles), oxythiamine (open circles), TMP (open triangles), and TPP (black squares). All error bars indicate the SD from three experiments.
