ΔG° of CLC-ec1 dimerization in 2:1 POPE/POPG lipid bilayers: A comparison of monomer and dimer benchmarks. Comparison of ΔG° values determined using the different methods of correcting for the nonreactive monomeric and dimeric signals: (1) monomer/dimer models using the 400-nm EPL liposome distribution from Walden et al., (2) monomer/dimer models using the 400 nm 2:1 POPE/POPG liposome distribution, (3) monomer (WW-Cy5) and dimer (WT-Cy5 + glutaraldehyde), and (4) monomer (WW-Cy5) and dimer (R230C/L249C-Cy5). Reported as best-fit ± error. All values are provided in Table 2.