Figure 6.
Figure 6. FDimer versus χ* analysis of W-Cy5. Comparison of experimental W-Cy5 photobleaching probabilities (top: P1, P2, P3+; red circles, mean ± SEM, n = 2–3; previously reported in Chadda et al., 2016) and FDimer versus χ* (bottom) against nonreactive monomer (gray) and dimer (black) models (PCy5 = 0.72, Pnon-specific = 0.14, bias = 4, and Alipid = 0.6) or experimental data. (A) The 400-nm EPL Walden liposome size distribution. (B) The 400-nm 2:1 POPE/POPG liposome size distribution. (C) WW-Cy5 for monomer and glutaraldehyde cross-linked WT-Cy5 for dimer. (D) WW-Cy5 for monomer and R230C/L249C-Cy5 for dimer.

FDimer versus χ* analysis of W-Cy5. Comparison of experimental W-Cy5 photobleaching probabilities (top: P1, P2, P3+; red circles, mean ± SEM, n = 2–3; previously reported in Chadda et al., 2016) and FDimer versus χ* (bottom) against nonreactive monomer (gray) and dimer (black) models (PCy5 = 0.72, Pnon-specific = 0.14, bias = 4, and Alipid = 0.6) or experimental data. (A) The 400-nm EPL Walden liposome size distribution. (B) The 400-nm 2:1 POPE/POPG liposome size distribution. (C) WW-Cy5 for monomer and glutaraldehyde cross-linked WT-Cy5 for dimer. (D) WW-Cy5 for monomer and R230C/L249C-Cy5 for dimer.

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