Figure 2.
Figure 2. WW-Cy5 is monomeric in 2:1 POPE/POPG membranes at χ* < 10−5 subunits/lipid. Comparison of experimental WW-Cy5 photobleaching probabilities (top: P1, P2, P3+; red circles, mean ± SEM, n = 2–3; previously reported in Chadda et al. [2016]) and FDimer versus χ* (bottom), against nonreactive monomer (gray) and dimer (black) predictions calculated using different liposome size distributions. (A) The 400-nm EPL Walden liposome size distribution. FDimer versus χ* shows an increase in dimerization at high densities and can be fit to a dimerization isotherm (ΔG° = −7.7 ± 0.3 kcal/mol, R2 = 0.30). (B) The 400-nm 2:1 POPE/POPG liposome size distribution. The updated liposome size distribution shows that FDimer does not increase indicating that WW-Cy5 is monomeric across the examined density range. Simulation parameters: PCy5 = 0.72, Pnon-specific = 0.14, bias = 4 and Alipid = 0.6.

WW-Cy5 is monomeric in 2:1 POPE/POPG membranes at χ* < 10−5 subunits/lipid. Comparison of experimental WW-Cy5 photobleaching probabilities (top: P1, P2, P3+; red circles, mean ± SEM, n = 2–3; previously reported in Chadda et al. [2016]) and FDimer versus χ* (bottom), against nonreactive monomer (gray) and dimer (black) predictions calculated using different liposome size distributions. (A) The 400-nm EPL Walden liposome size distribution. FDimer versus χ* shows an increase in dimerization at high densities and can be fit to a dimerization isotherm (ΔG° = −7.7 ± 0.3 kcal/mol, R2 = 0.30). (B) The 400-nm 2:1 POPE/POPG liposome size distribution. The updated liposome size distribution shows that FDimer does not increase indicating that WW-Cy5 is monomeric across the examined density range. Simulation parameters: PCy5 = 0.72, Pnon-specific = 0.14, bias = 4 and Alipid = 0.6.

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