Figure 1.

The 400-nm 2:1 POPE/POPG liposome size distribution. (A) 400-nm EPL liposome size distribution reported in Walden et al. (2007). (B) Fractional surface area (FSA) distribution. (C) Cryo-EM image of 400-nm extruded 2:1 POPE/POPG liposomes. Image has been enhanced for clear visualization of all liposomes (outlined in yellow), both in holes and on the carbon. (D) Raw cryo-EM image showing the structure of the lipid bilayer (dark lines). (E) The 400-nm 2:1 POPE/POPG liposome size distribution (mean ± SEM, n = 2). (F) FSA distribution. Actual values are reported in Table 1.

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