Relative permeability for large organic cations with respect to sodium. (A) Unitary current amplitudes (black circles) and open probability (red crosses) of TRPV1 in response to LPA relative to time. While the unitary current remains stable over time, the open probability increases with time until reaching a maximum after 3 min. (B) Representative traces elicited by voltage ramps (−120 to 120 mV) from inside-out membrane patches exposed to capsaicin (4 µM) for 1 min (solid black) and for 8 min (dashed black) and to LPA (5 µM) for 1 min (solid red) and 8 min (dashed red). The right graph zooms in the region of the curves where the E_rev occurs for all four conditions stated above. (C) Relative permeability of NMDG (∼4.5A°) in response to capsaicin (black; n = 3) or LPA (red; n = 10). Lines between the symbols indicate mean ± SEM. No statistical differences among permeabilities at the different times measured were found for this set of data; P 0.01.