Figure 2. The absence of CDF in Cav2.2 is not affected by alternative splicing of exon 37. (A–C) Left, representative ICa and IBa evoked before (P1, gray trace) and after (P2, red trace) a prepulse to 20 mV for Cav2.1 (A) and Cav2.2 variants with exon 37b (B) or exon 37a (C). Current traces were overlaid for comparison. Voltage protocol is shown above. P1 and P2 pulses were 10-ms steps from −80 mV to −5 mV (for ICa) or −10 mV (for IBa) 1 s before and 5 ms after, respectively, a 50-ms prepulse to various voltages. For P2 and P1, tail currents were resolved by repolarization to −60 mV for 2 ms before stepping to −80 mV. Right, the ratio of P2 and P1 tail currents is plotted against prepulse voltages for ICa and IBa. Numbers of cells for ICa and IBa are indicated in Table 1. Data represent mean ± SEM.
Figure 2.

The absence of CDF in Cav2.2 is not affected by alternative splicing of exon 37. (A–C) Left, representative ICa and IBa evoked before (P1, gray trace) and after (P2, red trace) a prepulse to 20 mV for Cav2.1 (A) and Cav2.2 variants with exon 37b (B) or exon 37a (C). Current traces were overlaid for comparison. Voltage protocol is shown above. P1 and P2 pulses were 10-ms steps from −80 mV to −5 mV (for ICa) or −10 mV (for IBa) 1 s before and 5 ms after, respectively, a 50-ms prepulse to various voltages. For P2 and P1, tail currents were resolved by repolarization to −60 mV for 2 ms before stepping to −80 mV. Right, the ratio of P2 and P1 tail currents is plotted against prepulse voltages for ICa and IBa. Numbers of cells for ICa and IBa are indicated in Table 1. Data represent mean ± SEM.

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