Figure 2. Effect of nebulin on tension and stiffness. (A) Tension and (B) stiffness in standard activation solution (pCa 4.55, 8 mM Pi, 5 mM MgATP, 200 mM ionic strength) at 25°C. n = 14 for Neb+ fibers and n = 22 for Neb− fibers. (C) Rigor stiffness measured at 100 Hz. Fibers were brought to the rigor condition directly from the standard activation solution by washing out ATP. n = 13 (Neb+ fibers) and n = 21 (Neb− fibers). In this and the other figures, data are shown as the mean and SEM.
Figure 2.

Effect of nebulin on tension and stiffness. (A) Tension and (B) stiffness in standard activation solution (pCa 4.55, 8 mM Pi, 5 mM MgATP, 200 mM ionic strength) at 25°C. n = 14 for Neb+ fibers and n = 22 for Neb− fibers. (C) Rigor stiffness measured at 100 Hz. Fibers were brought to the rigor condition directly from the standard activation solution by washing out ATP. n = 13 (Neb+ fibers) and n = 21 (Neb− fibers). In this and the other figures, data are shown as the mean and SEM.

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