Figure 4.
![Figure 4. Hill analysis of ramp data with varying spermine concentration. Positive ramps from −200 to 200 mV with varying internal spermine concentrations from different experiments. The control experiment is nominally free of spermine. The Hill-transformed conductances were fitted by eye to the COSB model in the intermediate region around V = 0 mV. The Gmin error factor b was set to zero. Adjusted variables are: [B] = 1.0 nM; ΔqL = 0.13 eo, VL = 46 mV, Δq1 = 3.8 eo, V1 = 8 mV, Δq2 = 1.5 eo, and V2 = 33 mV.](https://cdn.rupress.org/rup/content_public/journal/jgp/150/11/10.1085_jgp.201812127/5/jgp_201812127_fig4.jpeg?Expires=1767742546&Signature=dsciUQvx14QfQE~pIykdT9ZuuPmXrNOk8Vdn5AXqWIoaMWeGR8H3dQ2MYzbT2a1FxuEdB9NS9-HUcYA3y~T7ogmK0xR1irUBYmvXkxOuHNRkcpA-sjNQ1dje~JiZ5NAdJgaJcbRQG2dclpACsvVna3eOkD2OLcd8gtYck-FVSeXDtMCMSB0yEF3GscSRHjr27sATRy7NZcpfuqI0RDNVFPi9~JJdEUv8DtRZQVs8511SPPNQ2pwJGBBYWAbpwWn2CKBcdwDf6dEr10zWmhVCBJUk-w9udeKgbNVxgY4OLEPCMs1IGufvHQBIY0AYXRpGZVW49oMiPsVW8mwLLJ0lYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Hill analysis of ramp data with varying spermine concentration. Positive ramps from −200 to 200 mV with varying internal spermine concentrations from different experiments. The control experiment is nominally free of spermine. The Hill-transformed conductances were fitted by eye to the COSB model in the intermediate region around V = 0 mV. The Gmin error factor b was set to zero. Adjusted variables are: [B] = 1.0 nM; ΔqL = 0.13 eo, VL = 46 mV, Δq1 = 3.8 eo, V1 = 8 mV, Δq2 = 1.5 eo, and V2 = 33 mV.
