Diazoxide paradigm unmasks effect of CMA2 on triggering Ca²⁺ influx. (A–D) The MIN6 cells were treated 24 h with CMA2 (100 nM) and then prepared for Ca²⁺ influx measurements using Fura-PE3 (5 µM). (A) Cells were preincubated in KRBH without glucose for 1 h and loaded with Fura-PE3 for 30 min followed by a baseline Ca²⁺ measurement for 5 min. 20 mM glucose was added, and Fura fluorescence was measured for 30 min. (B) Diazoxide (250 µM) was included in the KRBH during the baseline reading and throughout the stimulation protocol. Data are shown as traces representing the mean ± SEM (shown as dots; n = 3). (C and D) Area under the curve (AUC) analyses for their respective traces. C shows data in A; D shows data in B. *, P < 0.05 CMA2 versus DMSO by one-way ANOVA.