Figure 4. Single-molecule colocalization between fluorescently tagged VSD and PD. (A) Mean colocalization coefficients ± SEM, after subtraction of random colocalization (n = 8–14); asterisk indicates statistical significance (P < 0.05 in t test with Bonferroni correction) with respect to the wild-type channel. Tagged full-length subunits were used as positive control. (B–E) Representative 13 × 13–µm images for each condition and mean numbers of fluorescent spots per image ± SEM. Bars, 2.5 µm.
Figure 4.

Single-molecule colocalization between fluorescently tagged VSD and PD. (A) Mean colocalization coefficients ± SEM, after subtraction of random colocalization (n = 8–14); asterisk indicates statistical significance (P < 0.05 in t test with Bonferroni correction) with respect to the wild-type channel. Tagged full-length subunits were used as positive control. (B–E) Representative 13 × 13–µm images for each condition and mean numbers of fluorescent spots per image ± SEM. Bars, 2.5 µm.

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