Figure 1. Constitutive activity of channels split within S4. (A) Lateral view of a KV10.1 subunit (PDB code 5K7L). The S4 helix is indicated in light blue, the S4–S5 linker in pink, the PD helices in gray, and the C linker in salmon. (Top right) KV10.1 sequence in the S4 helix and in the S4–S5 linker. We generated a series of split channels by disconnecting the VSD from the PD after every residue between the positions shown by red arrows. (Inset) The C-terminal S4 helix and the S4–S5 linker colored in a way corresponding with data points in C. (B) Representative, macroscopic currents of the wild-type channel and L341 split recorded without leak subtraction. (C) Normalized, mean current-voltage curves of the wild-type and several split channels (n = 6–14). Error bars denote SEM. (Inset) Normalized current at −100 mV plotted against the split position (mean ± SEM); dashed line shows the current level in the wild type.
Figure 1.

Constitutive activity of channels split within S4. (A) Lateral view of a KV10.1 subunit (PDB code 5K7L). The S4 helix is indicated in light blue, the S4–S5 linker in pink, the PD helices in gray, and the C linker in salmon. (Top right) KV10.1 sequence in the S4 helix and in the S4–S5 linker. We generated a series of split channels by disconnecting the VSD from the PD after every residue between the positions shown by red arrows. (Inset) The C-terminal S4 helix and the S4–S5 linker colored in a way corresponding with data points in C. (B) Representative, macroscopic currents of the wild-type channel and L341 split recorded without leak subtraction. (C) Normalized, mean current-voltage curves of the wild-type and several split channels (n = 6–14). Error bars denote SEM. (Inset) Normalized current at −100 mV plotted against the split position (mean ± SEM); dashed line shows the current level in the wild type.

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