Figure 5. PI(5)P is sufficient to activate GIRK2 channels in proteoliposomes. (a) Cartoon shows design of fluorescence-based K+ flux assay with GIRK2-containing liposomes. (b) Normalized mean traces of K+ flux for GIRK2 with acute application of CCCP, 30 µM of the indicated diC8 phosphoinositides (blue), and valinomycin. SEM bars are omitted for clarity. (c) Bar graph shows steady-state response of each phosphoinositide normalized to the response with PI(4,5)P2 and after subtracting basal flux (e.g., “Fractional activation vs. PI(4,5)P2”). Bars represent mean ± SEM. **, P < 0.05 ANOVA with Bonferroni post hoc test versus control (no diC8).
Figure 5.

PI(5)P is sufficient to activate GIRK2 channels in proteoliposomes. (a) Cartoon shows design of fluorescence-based K+ flux assay with GIRK2-containing liposomes. (b) Normalized mean traces of K+ flux for GIRK2 with acute application of CCCP, 30 µM of the indicated diC8 phosphoinositides (blue), and valinomycin. SEM bars are omitted for clarity. (c) Bar graph shows steady-state response of each phosphoinositide normalized to the response with PI(4,5)P2 and after subtracting basal flux (e.g., “Fractional activation vs. PI(4,5)P2”). Bars represent mean ± SEM. **, P < 0.05 ANOVA with Bonferroni post hoc test versus control (no diC8).

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