GIRK2 6′Y channels display altered association for PIP₂. (a–c) The current is plotted as a function of time for a cell expressing Dr-Vsp with GIRK2*-6′Y (a), GIRK2*-6’K (WT; b), or Kir2.1 (c) channels. V_h = −120 mV. Voltage-dependent (+100 mV) activation times for Dr-Vsp are indicated by orange bars. 100 mM 1-propanol, 100 mM ethanol, or 100 mM MPD (P, E, and M, respectively) and 3 mM Ba²⁺ were applied before and after Dr-Vsp activation. (d) Fractional inhibition of steady-state basal current is plotted as a function of different Dr-Vsp activation times. Note the rank order of Kir2.1 > GIRK2*-6′Y > GIRK2* depletion time. n = 7–10. (e) Representative examples of GIRK current depletion and recovery after 100 ms activation of Dr-Vsp at +100 mV. V_h = −120 mV. Best-fit single exponentials with time constants are shown. (f) Mean tau (s) for depletion and recovery of current following Dr-Vsp–dependent depletion. **, P < 0.05; ns, not significant; Student’s t test. Bars represent mean ± SEM.