Figure 2. A tyrosine substitution at 6′K increases agonist-independent basal current. (a) Structures of amino acids introduced at 6′K in GIRK2. (b) Current–voltage plots show the change in inwardly rectifying current with different alcohols and Ba2+ for 6′R and 6′Y. (c) Bar graph shows change in current density with different substitutions at 6’K. Note the significantly larger current for 6′Y (**, P < 0.05). (d) Mean percentage change in current, relative to the Ba2+ basal, for 100 mM ethanol (EtOH), 100 mM 1-propanol (PrOH), or MPD (100 mM) with different amino acid substitutions at the 6′ position. Note the reduced alcohol responses for 6′Y (n = 8–18). Bars represent mean ± SEM.
Figure 2.

A tyrosine substitution at 6′K increases agonist-independent basal current. (a) Structures of amino acids introduced at 6′K in GIRK2. (b) Current–voltage plots show the change in inwardly rectifying current with different alcohols and Ba2+ for 6′R and 6′Y. (c) Bar graph shows change in current density with different substitutions at 6’K. Note the significantly larger current for 6′Y (**, P < 0.05). (d) Mean percentage change in current, relative to the Ba2+ basal, for 100 mM ethanol (EtOH), 100 mM 1-propanol (PrOH), or MPD (100 mM) with different amino acid substitutions at the 6′ position. Note the reduced alcohol responses for 6′Y (n = 8–18). Bars represent mean ± SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal