Exogenous “gene trap” knock-in results in loss of ANO5. (a) Ano5 knockout construct. Briefly, a “gene trapping” element was inserted between exons 8 and 9 consisting of an exogenous splice acceptor followed by stop codons (Griffin et al., 2016). (b) Western blot of SDS-PAGE gel from lysates of quadriceps (Quad) or testis from 3-mo-old wild-type or Ano5 knockout mice.