![Figure 1. Cl−-driven trapping of Gluex in Scen. (A) Close-up view of the Cl−-binding site in cmCLC. The numbering of Gluex, Sercen, and Tyrcen corresponds to CLC-ec1. Green spheres are the Cl− ions. (B) Schematic representation of the trapping experiments. (C) Normalized time course of H+ transport mediated by CLC-ec1 in the presence of 70, 300, 500, 750, or 1,000 mM symmetrical [Cl−]. The asterisk denotes the addition of valinomycin to initiate the experiment. The dashed line represents an exponential fit of the time courses. Bar, 10 s. (D) Mean steady-state H+ uptake in 1–1,000 mM symmetrical [Cl−] normalized to the value in 300 mM Cl. Values are reported in Table 1; error bars are the SEM of the number of experiments indicated in Table 1.](https://cdn.rupress.org/rup/content_public/journal/jgp/149/4/10.1085_jgp.201611682/6/jgp_201611682_fig1.jpeg?Expires=1767772303&Signature=TuxzkIvzixci4YRZSLFhrpEGTLULBzaysBu2MR2ZRak0fPsV3R~SIayjspabP0pKypPrSaXuzqr1zm0YFRgp37pajBBrwtn7wDVe9V6Qc4ngqH4c~hDYiEeTvMm2kU0AzFHQOIQSYY0pGf-4QyDGxXfqcCsPY1TBfZxI8MH9E3BW3bFmVkapfZWJra6NfuTFTE3X-L5wZ-tMp9kVsvCXgQqvFpcnzqwReigX5iNh0SNXhf6~hUYTZ4oXkzE7AuFgnV95uuGaQyMrzkHmguFcSqeGTsYyWzjRRGHEleJAKuaBHSHumHaKX4DEkyAiKz2L3vGZcF2IS82agpXnwPYucw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cl−-driven trapping of Gluex in Scen. (A) Close-up view of the Cl−-binding site in cmCLC. The numbering of Gluex, Sercen, and Tyrcen corresponds to CLC-ec1. Green spheres are the Cl− ions. (B) Schematic representation of the trapping experiments. (C) Normalized time course of H+ transport mediated by CLC-ec1 in the presence of 70, 300, 500, 750, or 1,000 mM symmetrical [Cl−]. The asterisk denotes the addition of valinomycin to initiate the experiment. The dashed line represents an exponential fit of the time courses. Bar, 10 s. (D) Mean steady-state H+ uptake in 1–1,000 mM symmetrical [Cl−] normalized to the value in 300 mM Cl. Values are reported in Table 1; error bars are the SEM of the number of experiments indicated in Table 1.
