Kir6.1 transcript is present in primary human and mouse islets. qRT-PCR in mouse and human islets. (A and B) Representative traces of transcript amplification versus cycle number from mouse islets. (A) Insulin is represented in red, Kir6.2 in green, and Kir6.1 in blue. Actin was used as housekeeping gene and is shown in orange. Same color lines are duplicates from the same sample. (B) Comparative amplification traces for Kir6.2 (green) and Kir6.1 (blue) genes in WT and Kir6.1KO islets (n = 5 mice of each genotype, each sample was run in duplicates). Kir6.2 transcripts are present at similar levels in both WT and Kir6.1KO islets, but Kir6.1 transcript is only detected in WT islets and not in Kir6.1KO islets. (C) Each color represents traces for Kir6.1 transcript and the corresponding actin transcript in islets from independent human cadaveric organ donors. (D) Mean crossing threshold (Ct) for transcripts from mouse and human islets. Data represent mean ± SEM, n = 4 independent batches of human islets and n = 5 mice from each genotype. Samples were run in duplicates.